产品编号Y-0044英文名称Amyloid Beta (25-35) Peptide中文名称β淀粉样肽25-35别 名Amyloid beta Peptide (25-35); Beta-Amyloid (25-35); Amyloid beta-Protein Fragment 25-35; A beta 25-35; Beta Amyloid 25-35. Aβ淀粉样肽25-35; β淀粉样肽(25-35); Amyloid β (25-35); Aβ 25-35; β Amyloid (25-35); β Amyloid 25-35.理论分子量1.060kDa性 状Lyophilized powder序 列GSNKGAIIGLM纯化方法HPLC活性Yes保存条件Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles.产品介绍Aβ是老年性痴呆发病的始动因子,并证实引起毒性主要部位在第25-35氨基酸之间(Aβ25-35) ,Aβ25-35(Aβ25-35 peptide)具有神经毒性,可使海马和隔区神经元的存活率下降,继而发生神经元凋亡的形态学改变.
转铁蛋白产品简介英文名称 Transferrin CAS 11096-37-0 纯度 min.98% 外观(性状) 白色至浅黄色固体 储存条件 2-8℃ 有效期 2年 溶解性 10 mg/mL in water 规格 10mg 单位 支 产品说明: 转铁蛋白又名运铁蛋白 transferrin,TRF,siderophilin)是血浆中主要的含铁蛋白质,负责运载由消化管吸收的铁和由红细胞降解释放的铁。以 TRF-Fe3+的复合物形式进入骨髓中,供成熟红细胞的生成。 转铁蛋白是一种能传递铁离子的蛋白质。用于生化研究。 别名:铁传递蛋白 铁蛋白 人转铁蛋白 纯度:98% 溶解性:10 mg/mL in water 注意事项: 1. 我司生产的生化试剂如无特殊标注,基本为非无菌包装,若用于细胞实验,请提前做好预处理。 2. 一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 3. 产品信息仅供参考,如有疑问请致电 400-968-6088 咨询。 4. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。 5. 为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
Recombinant human IL-6 protein, C-His产品编号bs-41177P英文名称Recombinant human IL-6 protein, C-His中文名称重组人白介素6蛋白别 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6. ;Recombinant human IL-6 protein, C-His Specific References (1) | bs-41177P has been referenced in 1 publications.[IF=4.966] 222Xiaolong Zhang. et al. Baicalein inhibits non‐small‐cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment. Cancer Sci. 2020 Oct; 111(10): 3802–3812 other ; PubMed:32691974理论分子量15kDa检测分子量21 kDa性 状Lyophilized or Liquid浓 度>0.5 mg/ml物 种Human序 列30-212/212纯 度>95% as determined by SDS-PAGE纯化方法AC内毒素Not analyzed表达系统E.coli活性Not tested标签C-His缓 冲 液20 mM Tris-HCl (pH=8.0) with 150 mM NaCl保存条件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacterium Streptococcus pneumoniae.SWISS:P05231Gene ID:3569产品图片
Recombinant mouse IL-6 protein, C-His产品编号bs-10861P英文名称Recombinant mouse IL-6 protein, C-His中文名称重组小鼠白介素IL-6蛋白别 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6; Recombinant mouse IL-6 protein, C-His 理论分子量23kDa检测分子量26 kDa性 状Lyophilized or Liquid浓 度>0.5 mg/ml物 种Mouse序 列25-211/211纯 度>95% as determined by SDS-PAGE纯化方法AC内毒素Not analyzed表达系统E.coli活性Not tested标签C-His缓 冲 液20 mM Tris-HCl with 20% Glycerol保存条件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011].SWISS:P08505Gene ID:16193产品图片
Recombinant human IL-10 protein (Active, HEK293) 产品编号ck0006英文名称Recombinant human IL-10 protein (Active, HEK293)中文名称重组人白介素10 (活性的, Tag free)别 名Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. ;Recombinant human IL-10 protein (Active, HEK293) 人白介素10 重组蛋白; 人白细胞介素10 ; 重组人白介素10; 重组人IL-10;理论分子量17kDa检测分子量21 kDa性 状Lyophilized powder浓 度>0.5 mg/ml物 种Human序 列19-178/178纯 度>95% as determined by SDS-PAGE纯化方法AC内毒素<0.5 EU/μg as determined by LAL.表达系统HEK293 cell活性Yes标签Tag free缓 冲 液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存条件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍Interleukin 10(IL-10) is a member of the IL-10 family of α-helical cytokines. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of a number of proinflammatory cytokines such as IFNgamma, IL-2, IL-3, TNF and GM-CSF. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.This recombinant human IL-10 is produced by human cells.(Tag free)Biological activity:The activity was measured by its ability to stimulate the proliferation of MC/9-2 cells (mouse master cell line).Reconstitution:Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.SWISS:P18893Gene ID:3586产品图片
Recombinant human IL-15 protein (Active, HEK293) 产品编号ck0013产品分类重组蛋白英文名称Recombinant human IL-15 protein (Active, HEK293)中文名称重组人白介素15 (活性的, Tag free)英文别名rhIL-15; Interleukin-15; IL 15; Interleukin 15; Interleukin15; MGC9721; IL15; IL15_HUMAN. ;Recombinant human IL-15 protein (Active, HEK293)中文别名人白介素15重组蛋白; 重组人白介素15; 人重组白介素15;纯化方法AC检测分子量14-15 kDaGeneID3600SwissP40933浓度>0.5 mg/ml储存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存条件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationIsoform IL15-S48AA: Secreted. Isoform IL15-S21AA: Cytoplasm. Nucleus. Note=IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.Tissue SpecificityMost abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.SimilarityBelongs to the IL-15/IL-21 family.Database linksEntrez Gene: 3600 HumanOmim: 600554 HumanSwissProt: P40933 HumanUnigene: 654378 Human多肽活性Yes蛋白质与多肽-多肽序列49-162/162小分子偶联蛋白-蛋白载体标签Tag free纯度>95% as determined by SDS-PAGE内毒素<0.5 EU/μg as determined by LAL.重组蛋白-表达系统HEK293 cell复溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
产品编号ck0017产品分类重组蛋白英文名称Recombinant human IL-2 protein (Active, HEK293)中文名称重组人白介素2 (活性的, Tag free)英文别名Interleukin-2; Interleukin 2; Interleukin2; rhIL-2; T-cell growth factor; T Cell Growth Factor; Aldesleukin; IL 2; IL2; Lymphokine; POIL2; T Cell Growth Factor; TCGF; Involved in regulation of T cell clonal expansion; IL2_HUMAN. ;Recombinant human IL-2 protein (Active, HEK293)中文别名人白介素2重组蛋白; 重组人白介素2; 人重组白介素2; 重组人白介素2(IL-2);蛋白细胞定位分泌型蛋白纯化方法AC理论分子量15kDa检测分子量17 kDaGeneID3558SwissP60568浓度>0.5 mg/ml储存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存条件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationSecreted.DISEASENote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.SimilarityBelongs to the IL-2 family.Database linksEntrez Gene: 3558 HumanEntrez Gene: 16183 MouseEntrez Gene: 116562 RatOmim: 147680 HumanSwissProt: P60568 HumanSwissProt: P04351 MouseSwissProt: P17108 RatUnigene: 89679 HumanUnigene: 14190 MouseUnigene: 9871 Rat背景资料The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]多肽活性Yes蛋白质与多肽-多肽序列21-153/153小分子偶联蛋白-蛋白载体标签Tag free纯度>95% as determined by SDS-PAGE内毒素<0.5 EU/μg as determined by LAL.重组蛋白-表达系统HEK293 cell复溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
产品介绍 NCM Universal Antibody Diluent(通用型抗体稀释液)是一种即用型的,适合于稀释所 有免疫抗体测定(Immunofluorescence, IHC, ELISA, WB)实验中的抗体。该试剂可用于一抗 或二抗的稀释和配制,在4℃保存和使用不少于六个月;稀释液中加入了多种抗体结合反应 增强剂及稳定剂,增强免疫反应,减少非特异性结合,获得更佳的效果,可反复使用,节约 宝贵的抗体。试剂中不含有动物源的蛋白、磷酸盐、叠氮钠或硫柳汞类防腐剂,适合于所有 类型抗体稀释,包括 HRP,AP 标记的抗体。 产品特色 即用型稀释液,无需配制 适用所有抗体稀释(IF、IHC、ELISA、WB) 加入抗体结合反应增强剂,效果更佳 不含 BSA、脱脂奶粉等动物源蛋白 不含磷酸盐、叠氮钠或硫柳汞类防腐剂 操作步骤 根据抗体使用说明书,直接用本品对一抗或二抗进行适当倍数的稀释。保存在 NCM Universal Antibody Diluent 稀释液中的抗体可回收于 4℃保存,至少可反复多次使用 长达 6 个月以上,直至不能达到理想结果后丢弃。 保存条件 4℃保存,冰袋运输,至少 1年有效; 注意事项 1. 孵育抗体时尽量在 4 ºC 进行,可延长稀释后抗体的使用寿命; 2. 本品不含叠氮钠或硫柳汞类防腐剂,但含安全性更高的防腐剂,还需小心操作避免皮肤 直接接触。
蛋白酶K产品简介英文名称Proteinase KCAS39450-01-6酶活/效价40unit/mg dry weight单位支外观(性状)白色粉末至类白色粉末储存条件2-8℃有效期2年溶解性10mg/ml in water规格100mg 1g推荐浓度10mg/ml蛋白酶K是一种丝氨酸蛋白酶,因此可以用苯甲基磺酰氟抑制其作用,苯甲基磺酰氟是一种丝氨酸蛋白酶的抑制剂。蛋白酶K已广泛应用在皮革、毛皮、丝绸、医药、食品、酿造等方面。皮革工业的脱毛和软化已大量利用蛋白酶,既节省时间,又改善劳动卫生条件。蛋白酶还可用于蚕丝脱胶、肉类嫩化、酒类澄清。临床上可作药用,如用胃蛋白酶治疗消化不良,用酸性蛋白酶治疗支气管炎,用弹性蛋白酶治疗脉管炎以及用胰蛋白酶、胰凝乳蛋白酶对外科化脓性创口的净化及胸腔间浆膜粘连的治疗。加酶洗衣粉是洗涤剂中的新产品,含碱性蛋白酶,能去除衣物上的血渍和蛋白污物,但使用时注意不要接触皮肤,以免损伤皮肤表面的蛋白质,引起皮疹、湿疹等过敏现象。备注:产品信息可能会有优化升级。请以实际标签信息为准。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.蛋白酶K_酶&辅酶&抑制剂_生化试剂_索莱宝-专业生化试剂网上商城 (solarbio.com)
胶原酶V产品简介英文名称Collagenase ⅤCAS9001-12-1来源From clostridium histolyticum别名Clostridiopeptidase A酶活/效价125 CDU/mg sold单位瓶外观(性状)深褐色粉末储存条件-20℃有效期2年溶解性5 mg/mlWater规格100mg 1g胶原酶Ⅴ包含至少7种蛋白酶成分,分子量从68-130KD不等。它可用于胰腺小岛组织的分离,将结缔组织分离成单个细胞。溶解性:可溶于TES缓冲液,Hank's平衡盐和Earles平衡盐等溶液。溶液在-20℃可稳定保存数月。胶原酶Ⅴ(来源溶组织梭菌)包含至少 7 种蛋白酶成分,分子量从 68-130KD 不等。它可用于胰腺小岛组织的分离, 将结缔组织分离成单个细胞。消化软骨组织的浓度一般为 1-2 mg/ml,消化肝脏组织大约 1 mg/ml。 但是具体的消化浓度因种属特异性而异。很多文献指出胶原酶可以消化多种组织,胶原酶的使用浓 度一般为 0.1- 5 mg/ml,消化过程中可以搅拌以促进酶的消化作用。胶原酶在消化的过程中可以引起 细胞的少量死亡,一般消化时间为 15min 至数小时。如果浓度发生变化那么消化时间可能更长。消化过程中首选的缓冲液为含钙离子和 BSA 的 Krebs Ringer Buffer。 在消化的过程中如果细胞大量死亡则需要降低酶量,同时可以加入0.5%BSA或5-10%血清来稳定细胞的状态和消化效果。胶原酶的灭菌首先离心或者用0.8µm的滤器去掉不溶物。然后再用0.2µm 的滤器过滤除菌。 对于组织培养方面的应用,胶原酶可以用不含钙离子的溶液配制如hanks,ebss等。0.05 - 0.1 mg/ml 胶原酶溶于50 mM TES buffer,pH 7.4 (37 ℃) 含 0.36 mM 氯化钙。反应体系 的终浓度为50 mM TES,0.36 mM 氯化钙,25 mg 胶原,0.005-0.01 mg 胶原酶。酶液在含有 0.3-0.5 mM 钙离子的中性 PH 条件下可以在 37 ℃维持活性 15 小时。在-20℃可以维 持数月。 备注:产品信息可能会有优化升级。请以实际标签信息为准。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.胶原酶V_酶&辅酶&抑制剂_生化试剂_索莱宝-专业生化试剂网上商城 (solarbio.com)
胶原酶III产品简介CAS9001-12-1英文名称CollagenaseIII别名梭菌肽酶 A酶活/效价290u/mg单位支外观(性状)褐色冻干粉储存条件-20℃有效期2年溶解性5 mg/mlWater规格100mg 1g 5g用于消化组织中连接部分使其成为单个细胞,用于哺乳动物组织细胞解离胶原蛋白酶 来源于溶组织梭菌Collagenase from Clostridium histolyticumpurified by chromatography, Type III, 2-10 FALGPA units/mg solid, ≥250 CDU/mg solid (CDU = collagen digestion units), lyophilized powderBiochem/physiol ActionsCollagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.Crude collagenases are mixtures of enzymes (mostly proteases) secreted by C. histolyticum. Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa.包装Product sold on the basis of collagen digestion units (CDU).Preparation NoteCollagenase may be dissolved in TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4 at 37 °C) at 0.05-0.1 mg/mL concentration.Unit DefinitionOne collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.ApplicationCollagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. It may be used for the disaggregation of human tumor, mouse kidney, human adult/fetal brain, lung and many other tissues. It may also be used in liver and kidney perfusion studies, for the digestion of pancreas, isolation of nonparenchymal rat liver cells and for hepatocyte preparation.type Type IIIform lyophilized powderpurified by chromatographysolubility TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble0.05-0.1 mg/mL at 37 °Cforeign activity Clostripain, may contain trace amount neutral protease<1 unit/mg solidstorage temp. −20°C 备注:产品信息可能会有优化升级。请以实际标签信息为准。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.胶原酶III_酶&辅酶&抑制剂_生化试剂_索莱宝-专业生化试剂网上商城 (solarbio.com)
胶原酶Ⅱ产品简介CAS9001-12-1英文名称CollagenaseⅡ来源Prepared from Cl.histolyticum别名梭菌肽酶 A酶活/效价≥125units/mg solid单位支外观(性状)褐色粉末有效期2年储存条件-20℃溶解性5 mg/mlWater规格100mg 1g胶原酶Ⅱ适用于脂肪、肝脏,骨,甲状腺,心脏和唾液腺组织细胞的分离。胶原蛋白酶 来源于溶组织梭菌胶原酶Ⅱ可以用 TESCA buffer (50 mM TES,0.36 mM 氯化钙,pH 7.4,37°C)配制成 1-2 mg/ml。 也可以用含钙镁的 PBS、含钙镁的 hanks 或不含血清的培养基配制。配完后分装,避免反复冻融。1 个胶原消化单位(CDU):在 pH 7.4,37℃以及钙离子存在的条件下,以每5小时从胶原中 释放的多肽相当于茚三酮显色1微摩尔亮氨酸量为一个活性单位。 1FALGPA 单位:在 pH 7.5、25℃以及钙离子存在的条件下,一个 FALGPA 水解单位每分钟 可水解 1.0µmol 呋喃基丙烯酰基-Leu-Gly-Pro-Ala。胶原酶Ⅱ为粗品,用于分离脂肪细胞,也可以用于分离各种组织、肿瘤,尤其是上皮组织。外 观为黑棕色粉末。分子量68,000到125,000,最适PH为6.3-8.8。胶原酶能够特异性结合 -R-Pro-8-X-Gly-Pro-R-序列中的中性氨基酸(X)和甘氨酸之间的肽键。该粗品是溶组织梭菌分泌的 混合酶。主要为胶原酶、中性蛋白酶和梭菌蛋白酶。其中梭菌蛋白酶是被氧化的、最不活泼的酶, 组织的分离必须依靠胶原酶和中性蛋白酶两者共同作用。激活剂:Ca2+,每摩尔酶需要4g钙离子激活。抑制剂:EGTA、还原性谷胱甘肽、β-巯基乙醇、巯基乙酸钠、8-羟基喹啉、2,2'-联吡啶等。底物:不同类型的胶原是其天然底物,而一些合成的多肽也可以作为其底物,比如: N-CBZ-gly-pro-gly-gly-pro-ala (Km = 0.71 mM)、N-CBZ-gly-pro-leu-gly-pro、N-(3-(2-furyl)acryloyl)- leu-gly-pro-ala(FALGPA)、4-Phenylazo benzyloxycarbonyl-pro-leu-gly-pro-D-arg、N-2,4-Dinitrophenyl- pro-gln-gly-ile-ala-gly-gln-D-arg。除此之外N-Succinyl-gly-pro-leu-glypro7-amido-4-methylcoumarin是 类胶原酶的肽酶的底物。N-(2,4-Dinitrophenyl)-proleu-gly-leu-trp-ala-D-arg 氨基酸化合物是脊椎动物 胶原酶的底物。 备注:产品信息可能会有优化升级。请以实际标签信息为准。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.胶原酶Ⅱ_酶&辅酶&抑制剂_生化试剂_索莱宝-专业生化试剂网上商城 (solarbio.com)
胶原酶Ⅳ产品简介CAS9001-12-1英文名称CollagenaseⅣ来源From clostridium histolyticum别名梭菌肽酶 A酶活/效价≥160units/mg solid单位支外观(性状)褐色冻干粉有效期2年储存条件-20℃溶解性10 mg/mlWater规格100mg 1g胶原酶Ⅳ包含至少7中蛋白酶成分,分子量从68-130KD不等。它能消化多种组织胶原蛋白酶,来源于溶组织梭菌。胶原酶Ⅳ包含至少7种蛋白酶成分,分子量从68-130KD不等。它能消化多种组织。胶原酶的使 用浓度一般为0.1- 5 mg/ml,消化过程中可以搅拌以促进酶的消化。胶原酶在消化的过程中可以引起 细胞的少量死亡,一般消化时间为15min至数小时。如果浓度发生变化那么消化时间可能更长。消 化过程中首选的缓冲液为含钙离子和BSA的Krebs Ringer Buffer。在消化的过程中如果细胞大量死亡 则需要降低酶量,同时可以加入0.5%BSA或5-10%血清来稳定细胞的状态和消化效果。 胶原酶的灭菌首先离心或者用0.8µm的滤器去掉不溶物。然后再用0.2µm的滤器过滤除菌。盐酸 苯甲脒和TLCK联合作用可以与该酶成分中大部分的蛋白酶结合而抑制其活性,但是他们却不会抑 制胶原酶的活性。其中盐酸苯甲脒的浓度一般为1 mM,TLCK 的浓度一般为0.100-0.135 mM。值得 注意的是两者不能完全保证所有蛋白酶的活性均被抑制。酶的溶解性:0.05 - 0.1 mg/ml 胶原酶溶于50 mM TES buffer,pH 7.4 (37 ℃) 含 0.36 mM 氯化 钙。反应体系的终浓度为50 mM TES,0.36 mM 氯化钙,25 mg 胶原,0.005-0.01 mg 胶原酶。酶液在含有 0.3-0.5 mM 钙离子 PH 7.5 的条件下可以在 37 ℃维持活性 5 小时。在-20℃可以维 持数月。 备注:产品信息可能会有优化升级。请以实际标签信息为准。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.胶原酶Ⅳ_酶&辅酶&抑制剂_生化试剂_索莱宝-专业生化试剂网上商城 (solarbio.com)
胶原酶Ⅰ产品简介CAS9001-12-1英文名称CollagenaseⅠ来源Collagenase from Clostridium histolyticum别名梭菌肽酶 A酶活/效价≥125units/mg solid单位支外观(性状)褐色结晶性粉末储存条件-20℃有效期2年规格100mg 1g胶原酶Ⅰ用于上皮、肺和肾上腺组织细胞的分离胶原蛋白酶 来源于溶组织梭菌胶原酶Ⅰ可以用 TESCA buffer (50 mM TES,0.36 mM 氯化钙,pH 7.4,37°C)配制成 1-2 mg/ml。 也可以用含钙镁的 PBS、含钙镁的 hanks 或不含血清的培养基配制。配完后分装,避免反复冻融。1 个胶原消化单位(CDU):在 pH 7.4,37℃以及钙离子存在的条件下,以每5小时从胶原中 释放的多肽相当于茚三酮显色1微摩尔亮氨酸量为一个活性单位。1FALGPA 单位:在 pH 7.5、25℃以及钙离子存在的条件下,一个 FALGPA 水解单位每分钟 可水解 1.0μmol 呋喃基丙烯酰基-Leu-Gly-Pro-Ala。胶原酶Ⅰ为粗品,用于上皮、肺和肾上腺组织细胞的分离。外观为黑棕色粉末。分子量68,000 到125,000,最适PH为6.3-8.8。胶原酶能够特异性结合-R-Pro-8-X-Gly-Pro-R-序列中的中性氨基酸(X) 和甘氨酸之间的肽键。该粗品是溶组织梭菌分泌的混合酶。主要为胶原酶、中性蛋白酶和梭菌蛋白 酶。其中梭菌蛋白酶是被氧化的、最不活泼的酶,组织的分离必须依靠胶原酶和中性蛋白酶两者共 同作用。激活剂:Ca2+,最适合浓度为5 mM。抑制剂:EGTA、还原性谷胱甘肽、β-巯基乙醇、巯基乙酸钠、8-羟基喹啉、2,2'-联吡啶等。底物:不同类型的胶原是其天然底物,而一些合成的多肽也可以作为其底物,比如: N-CBZ-gly-pro-gly-gly-pro-ala (Km = 0.71 mM)、N-CBZ-gly-pro-leu-gly-pro、N-(3-(2-furyl)acryloyl)- leu-gly-pro-ala(FALGPA)、4-Phenylazo benzyloxycarbonyl-pro-leu-gly-pro-D-arg、N-2,4-Dinitrophenyl- pro-gln-gly-ile-ala-gly-gln-D-arg。除此之外N-Succinyl-gly-pro-leu-glypro7-amido-4-methylcoumarin是 类胶原酶的肽酶的底物。N-(2,4-Dinitrophenyl)-proleu-gly-leu-trp-ala-D-arg 氨基酸化合物是脊椎动物 胶原酶的底物。 备注:产品信息可能会有优化升级。请以实际标签信息为准。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.胶原酶Ⅰ_酶&辅酶&抑制剂_生化试剂_索莱宝-专业生化试剂网上商城 (solarbio.com)
牛血清白蛋白V (Elisa专用)产品简介CAS9048-46-8单位瓶纯度98%外观(性状)白色冻干粉有效期3年储存条件2-8℃溶解性100mg/ml in Water规格5g 25g 备注:产品信息可能会有优化升级。请以实际标签信息为准。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.
牛血清白蛋白 BSA(全组分)产品简介CAS9048-46-8来源牛血清英文名称Bovine Serum Albumin别名BSA 牛白 白蛋白(来源于牛血清)单位瓶纯度≥96%外观(性状)白色至淡黄色冻干粉末储存条件2-8℃有效期5年溶解性100mg/ml in Water规格5g 10g 25g 100g进口原料含量:≥98%氮含量:15.5 - 16.5 %pH: 6.7 - 7.3物理性状:白色至微黄色干粉或结晶;溶于水,溶解后由澄清变为略微浑浊,颜色由浅黄色变为黄绿色,参考浓度为40 mg/mL。用途:生化试剂,蛋白质及衍生物。遗传工程和医药研究,用作胎酶和其他蛋白质的稳定剂,蛋白质标准。牛血清白蛋白,英文名称为Bovine Serum Albumin,也称Bovine albumin,或Cohn Fraction V,简称BSA。BSA有着极其广泛的实验室应用,在免疫实验中常用作封闭剂,包括ELISA、WB(Western Blot)。作为载体蛋白,将其交联于半抗原和其他弱抗原可以使它们在抗体生产中具有更强的免疫原性。在限制性内切酶消化反应中,BSA常常被用作一些酶的反应稳定剂,并且能防止它粘附到管壁和枪头上。BSA也常用于生物制药的生产过程或者作为营养物用于细胞和微生物培养。除此之外,还作为蛋白定量检测的标准品使用。我们提供的BSA产品,使用优质牛血浆,利用热休克法(Heat Shock)法制备而成。本级别的牛血清白蛋白(BSA, Standard Grade),可以满足大部分常规实验需求,如用作免疫封闭剂、组织细胞培养添加剂、蛋白/酶稳定剂以及蛋白定量标准品。除了BSA-全,我司还提供其他应用级别的BSA,包括BSA-V(Cat: A8020),无脂肪酸(Cat: A8850),细胞培养级(Cat:H1130),BSA标准品(Cat: PC0001),BSA封闭液(Cat: SW3015)。注意事项: (1)用于细胞实验,溶解后,须用一次性针头滤器过滤除菌。(2)为了您的安全和健康,请穿实验服并戴一次性手套操作。 备注:产品信息可能会有优化升级。请以实际标签信息为准。 备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.牛血清白蛋白 BSA(全组分)_氨基酸&多肽&蛋白质_生化试剂_索莱宝-专业生化试剂网上商城 (solarbio.com)
产品编号bs-41494P英文名称Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R), N-His中文名称重组SARS冠状病毒2核衣壳突变体蛋白Omicron(P13L、E31del、R32del、S33del、R203K、G204R)别 名Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R), N-His 理论分子量45.3kDa检测分子量50 kDa性 状Lyophilized or Liquid浓 度>0.5 mg/ml物 种SARS-Cov-2序 列1-419/419纯 度>90% as determined by SDS-PAGE纯化方法AC内毒素Not analyzed表达系统E.coli活性Not tested标签N-His缓 冲 液10mM PB (pH=7.4) with 50mM Nacl保存条件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.SWISS:P0DTC9Gene ID:43740575产品图片The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
溶菌酶产品简介CAS9001-63-2单字母RVVRDPQGIRAWVAWRNR三字母Arg-Val-Val-Arg-Asp-Pro-Gln-Gly-Ile-Arg-Ala-Trp-Val-Ala-Trp-Arg-Asn-Arg别名Lysozyme;Muramidase分子式C99H159N37O23分子量2235.59纯度≥95%外观与性状冻干粉;Lyophilized powder盐体系TFA盐;Trifluoroacetate salt来源合成;Synthetic储存条件-20℃有效期2年单位瓶简介Lysozyme 是一种动物体内产生的的先天免疫系统的组分,具有杀菌作用。Lysozyme is an antimicrobial enzyme produced by animals that forms part of the innate immune system.参考文献[1] Feingold DS, et al. Locus of the action of serum and the role of lysozyme in the serum bactericidal reaction. J Bacteriol. 1968 Dec;96(6):2118-26.类别/标签其他规格1mg 5mg备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.溶菌酶_其他_多肽_索莱宝-专业生化试剂网上商城 (solarbio.com)
脱氧核糖核酸酶Ⅰ(DnaseⅠ)产品简介英文名称DNase I(DeoxyribonueleaseⅠ)CAS9003-98-9别名DNase I 脱氧核糖核酸 5′-寡核苷酸-水解酶酶活/效价1500 units/mg单位支分子量约31 kDa外观(性状)白色冻干粉储存条件-20℃有效期3年溶解性0.15 M NaCl (5mg/ml)规格25mg 100mg 1g说明:生化研究,催化脱氧核糖核酸降解,可用于蛋白或RNA的提取中去除DNA。酶反应如下:脱氧核糖核酸→二核苷酸-5’-磷酸+寡聚核苷酸-5’-磷酸物理性状:白色冻干粉,溶于无菌水,参考浓度5mg/mL。 备注:产品信息可能会有优化升级。请以实际标签信息为准。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.